Isolation and Partial Purification of Hepatic Stimulatory Substance.

نویسندگان

  • A Francavilla
  • P Ove
  • L Polimeno
  • M Coetzee
  • L Makowka
  • M Barone
  • D H Van Thiel
  • T E Starzl
چکیده

THE PURPOSE of this study was to purify and characterize hepatic stimulatory substance (HSS), a protein found in the liver that has been reported to stimulate hepatic DNA synthesis and hepatocyte replication and that was previously identified by LaBrecque et al l -3 and Francavilla et al in the rat liver,4,5 as well as by Starzl et al in the canine liver.6,7 HSS has been partially purified by successive steps, involving ethanol precipitation, ultrafiltration, and fast protein lipid chromatography (FPLC). The activity of this factor has been tested in 40% hepatectomized rats.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Further studies on hepatic stimulatory substance (SS) after partial hepatectomy.

The stimulatory substance (SS) that IS found In the cytosol of regenerating dog livers and which promoted regeneration when Injected into the portal vein after Eck fistula did not cause glomerular proliferation when active cytosol was injected into the renal artery, The finding was compatible with but did not prove organ specificity of hepatic SS, The development of SS was completely prevented ...

متن کامل

The effect of hepatic stimulatory substance, isolated from regenerating hepatic cytosol, and 50,000 and 300,000 subfractions in enhancing survival in experimental acute hepatic failure in rats treated with D-galactosamine.

Galactosamine induces a dose-dependent hepatic injury in rats and many other animals. The toxicity of D-galactosamine appears to be a consequence of the loss of hepatic UTP. It has previously been reported that regenerating liver cytosol is able to prevent, at least in part, the lethal effect of this substance by stimulating hepatic regeneration. Recently, we have separated a fraction using alc...

متن کامل

Effect of culture origin on chemical stimulation of sexual reproduction in Phytophthora and Pythium

Lecithin washed with NaCl solution was stimulatory to oospore formation of Phytophthora boehmeriae, P. sojae, P. cactorum, and Pythium aphanidermatum. Commercial glycerides of palmitic acid with 99% purity were found to contain a substance inhibitory to oospore formation. Purification with florisil column chromatography was more effective in removing the inhibitory substance than thin-layer chr...

متن کامل

Serum Vasoconstrictor (serotonin)

In the first paper of this series (1) the partial purification of a vasoconstrictor substance from beef serum was reported. Further purification of the active substance led to its isolation. A preliminary report of the method together with analytical and pharmacological data has also been published (2). It is the purpose of this paper to present the details of the method of isolation, the more ...

متن کامل

Effect on the canine Eck fistula liver of intraportal TGF-beta alone or with hepatic growth factors.

Transforming growth factor-beta canceled the hepatocyte proliferation caused by transforming growth factor-alpha when the two substances were mixed and administered through a disconnected central portal vein branch after creation of an Eck fistula. In contrast, transforming growth factor-beta had no antidotal action on the stimulatory effects of insulin or full test doses of insulinlike factor-...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Transplantation proceedings

دوره 20 1 Suppl 1  شماره 

صفحات  -

تاریخ انتشار 1988